Solid Tissue Task Force

To date, most analyses on the role of EVs have relied on studying vesicles from fluid, such as conditioned cell culture media, or body fluids. More recently methods have developed to isolate EVs from solid tissues such as brain and tumour tissue. Relative to the routine isolation of exosomes from extracellular fluids, such as conditioned cell culture media, blood, urine or CSF, many technical issues must be overcome to limit co-isolation of vesicles with the same physical and/or molecular properties as EVs. This taskforce has been formed to help share methodology, review current practices and develop guidelines for the isolation, characterization and study of EV isolated from solid tissue.