Cell culture-conditioned medium-derived extracellular vesicles (CCM-EVs)
About
To maximize the reliability and reproducibility of CCM-EV research, transparent reporting is needed to produce and characterize CCM-EVs. Our goals are to increase awareness and promote reproducibility in EV research when CCM is the starting material. In this task force, we aim to identify relevant cell culturing parameters, describe their effects based on current knowledge, recommend reporting parameters, and identify outstanding questions.
Working Groups
- Considerations for minimal criteria required for reporting cell culturing parameters
- Parasite cell culturing and their effects on EV release
- High throughput characterization
 Chair Contact Info
 Members
Working Group 1: Ana Claudia Torrecilhas | Bernd Giebel | Carolina Soekmadji | Carlos Salomon | Clotilde Théry | Dandan Zhu | Esther Nolte-'t Hoen | Faisal Alibhai | Faezeh Shekari | Francesca Perut | Gabriella Pocsfalvi | Ganesh Vilas Shelke | Ghasem Hosseini Salekdeh | Hossein Baharvand | Inge Nelissen | Kenneth Witwer | Lorena Martin-Jaula | Lorraine O'Driscoll | Mario Gimona | Owen Davies | Rienk Nieuwland | Simon Staubach | Stefania Bruno | Stefano Pluchino | Suresh Mathivanan | Tobias Tertel | Verena Börger
Working Group 2: Ana Claudia Torrecilhas (contact person) | Iris Aparici Herraiz | Carmen Becerra | Nuria Cortes-Serra | Hernando Del Portillo | Melisa Gualdron-Lopez | Antonio Marcilla | Christian M. Sánchez-López | Martin Olivier | Sima Raafati | Neta Regev-Rudzki | Mehrdad Shokouhy | Patricia Xander | Christopher Fernandez Prada
Working Group 3: Ghasem Hosseini Salekdeh | Suresh Mathiavan
 Publications
- Cell culture-derived extracellular vesicles: Considerations for reporting cell culturing parameters
Faezeh Shekari, Faisal J. Alibhai, Hossein Baharvand, Verena Börger, Stefania Bruno, Owen Davies, Bernd Giebel, Mario Gimona, Ghasem Hosseini Salekdeh, Lorena Martin-Jaular, Suresh Mathivanan, Inge Nelissen, Esther Nolte-’t Hoen, Lorraine O'Driscoll, Francesca Perut, Stefano Pluchino, Gabriella Pocsfalvi, Carlos Salomon, Carolina Soekmadji, Simon Staubach, Ana Claudia Torrecilhas, Ganesh Vilas Shelke, Tobias Tertel, Dandan Zhu, Clotilde Théry, Kenneth Witwer, Rienk Nieuwland
e115 First Published: 16 October 2023 - https://doi.org/10.1002/jex2.115
- Guidelines for the purification and characterization of extracellular vesicles of parasites
Carmen Fernandez-Becerra, Patrícia Xander, Daniel Alfandari, George Dong, Iris Aparici-Herraiz, Irit Rosenhek-Goldian, Mehrdad Shokouhy, Melisa Gualdron-Lopez, Nicholy Lozano, Nuria Cortes-Serra, Paula Abou Karam, Paula Meneghetti, Rafael Pedro Madeira, Ziv Porat, Rodrigo Pedro Soares, Adriana Oliveira Costa, Sima Rafati, Anabela-Cordeiro da Silva, Nuno Santarém, Christopher Fernandez-Prada, Marcel I. Ramirez, Dolores Bernal, Antonio Marcilla, Vera Lucia Pereira-Chioccola, Lysangela Ronalte Alves, Hernando Del Portillo, Neta Regev-Rudzki, Igor Correia de Almeida, Sergio Schenkman, Martin Olivier, Ana Claudia Torrecilhas https://doi.org/10.1002/jex2.117
 Other Outputs
- Initially, we provided a list of key cell culturing parameters that may affect EV release, biochemical composition, or therapeutic potency
- Task Force representation at EVClub (March 30, 2022)
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